Aravin, A. A., Sachidanandam, R., Girard, A., Fejes-Toth, K., Hannon, G. J. (May 2007) Developmentally regulated piRNA clusters implicate MILI in transposon control. Science, 316 (5825). pp. 744-7. ISSN 1095-9203 (Electronic)
Abstract
Nearly half of the mammalian genome is composed of repeated sequences. In Drosophila, Piwi proteins exert control over transposons. However, mammalian Piwi proteins, MIWI and MILI, partner with Piwi-interacting RNAs (piRNAs) that are depleted of repeat sequences, which raises questions about a role for mammalian Piwi's in transposon control. A search for murine small RNAs that might program Piwi proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila. We also find evidence of an adaptive amplification loop in which MILI catalyzes the formation of piRNA 5' ends. Mili mutants derepress LINE-1 (L1) and intracisternal A particle and lose DNA methylation of L1 elements, demonstrating an evolutionarily conserved role for PIWI proteins in transposon suppression.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | 3' Untranslated Regions Animals Cluster Analysis Computational Biology DNA Methylation Genes, Intracisternal A-Particle Long Interspersed Nucleotide Elements Male Meiosis Mice Mutation Proteins/ metabolism RNA, Antisense genetics metabolism RNA, Untranslated genetics/metabolism Retroelements Reverse Transcriptase Polymerase Chain Reaction Short Interspersed Nucleotide Elements Spermatocytes cytology/ metabolism Spermatogenesis Suppression, Genetic |
| Subjects: | organism description > animal > insect > Drosophila bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Piwi protein organism description > animal > mammal > rodent > mouse bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab CSHL labs > Sachidanandam lab School of Biological Sciences > Publications |
| Depositing User: | CSHL Librarian |
| Date: | 4 May 2007 |
| Date Deposited: | 02 Dec 2011 16:16 |
| Last Modified: | 22 Sep 2014 14:11 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22959 |
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