Komai, S., Licznerski, P., Cetin, A., Waters, J., Denk, W., Brecht, M., Osten, P.
(September 2006)
Postsynaptic excitability is necessary for strengthening of cortical sensory responses during experience-dependent development.
Nature Neuroscience, 9 (9).
pp. 1125-33.
ISSN 1097-6256
Abstract
Sensory experience is necessary for normal cortical development. This has been shown by sensory deprivation and pharmacological perturbation of the cortex. Because these manipulations affect the cortical network as a whole, the role of postsynaptic cellular properties during experience-dependent development is unclear. Here we addressed the developmental role of somatodendritic excitability, which enables postsynaptic spike timing-dependent forms of plasticity, in rat somatosensory cortex. We used short interfering RNA (siRNA)-based knockdown of Na+ channels to suppress the somatodendritic excitability of small numbers of layer 2/3 pyramidal neurons in the barrel cortex, without altering the ascending sensory pathway. In vivo recordings from siRNA-expressing cells revealed that this manipulation interfered with the normal developmental strengthening of sensory responses. The sensory responsiveness of neighboring cortical neurons was unchanged, indicating that the cortical network was unchanged. We conclude that somatodendritic excitability of the postsynaptic neuron is needed for the regulation of synaptic strength in the developing sensory cortex.
| Item Type: |
Paper
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| Uncontrolled Keywords: |
Animals
DNA Recombinant
administration dosage
genetics
Excitatory Postsynaptic Potentials
physiology
Green Fluorescent Proteins
genetics/metabolism
Lentivirus
genetics
Microscopy
Confocal
Neuronal Plasticity
physiology
Pyramidal Cells
metabolism
physiology
RNA Interference
physiology
RNA Small Interfering
genetics
metabolism
Rats
Rats, Wistar
Sensory Deprivation/physiology
Sodium Channels/genetics/*physiology
Somatosensory Cortex/*physiology
Synaptic Transmission/physiology |
| Subjects: |
organs, tissues, organelles, cell types and functions > tissues types and functions > barrel cortex
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > calcium channel
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Osten lab |
| Highlight: |
Osten, Pavel |
| Depositing User: |
CSHL Librarian
|
| Date: |
September 2006 |
| Date Deposited: |
13 Dec 2011 16:40 |
| Last Modified: |
18 Nov 2014 16:31 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/22843 |
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