Gao, M., Li, G., Potter, D., McCombie, W. R., Quiros, C. F. (2006) Comparative analysis of methylthioalkylmalate synthase (MAM) gene family and flanking DNA sequences in Brassica oleracea and Arabidopsis thaliana. Plant Cell Reports, 25 (6). pp. 592-598. ISSN 07217714
Abstract
Gene BoGSL-PRO is associated with presence of 3-carbon side-chain glucosinolates (GSL). This gene is a member of the methylthioalkylmalate synthase (MAM) gene family. A BAC clone of Brassica oleracea, B21F5, containing this gene, was sequenced, annotated and compared to its corresponding region in Arabidopsis thaliana. Twelve protein-coding genes and 10 transposable elements were found in this clone. The corresponding region in A. thaliana chromosome I has 14 genes and no transposable elements. Analysis of MAM gene family in both species, which also include genes controlling 4-carbon side-chain GSL, separated the genes in two groups based on exon numbers and function. Phylogenetic analysis of the amino acid sequences encoded by these genes suggest that these two groups were produced by a duplication that must have occurred before the divergence of the Rosid and Asterid lineages of angiosperms. Comparison with putative orthologs from several prokaryotes further suggest that the members of the gene family with 10 exons, which encode proteins involved in 4-carbon side-chain GSL biosynthesis, were derived via truncation of the 3? end from ancestral genes more similar in length to those with 12 exons, which encode proteins involved in 3-carbon side-chain GSL biosynthesis. Lower gene density in B. oleracea compared to A. thaliana is due in part to presence of transposable elements (TE) mostly in inter-genic regions. © Springer-Verlag 2005.
| Item Type: | Paper |
|---|---|
| Subjects: | organism description > plant > Arabidopsis bioinformatics > genomics and proteomics > annotation > sequence annotation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > glucosinolates |
| CSHL Authors: | |
| Communities: | CSHL labs > McCombie lab |
| Depositing User: | CSHL Librarian |
| Date: | 2006 |
| Date Deposited: | 19 Dec 2011 15:30 |
| Last Modified: | 11 Apr 2013 19:45 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22794 |
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