Akerman, C. J., Cline, H. T. (May 2006) Depolarizing GABAergic conductances regulate the balance of excitation to inhibition in the developing retinotectal circuit in vivo. J Neurosci, 26 (19). pp. 5117-30. ISSN 1529-2401 (Electronic)
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Depolarizing-GABAergic-conductances-regulate-the-balance-of-excitation-to-inhibition-in-the-developing-retinotectal-circuit-in-vivo_2006_Journal-of-Neuroscience.pdf - Published Version Download (2MB) | Preview |
Abstract
Neurotransmission during development regulates synaptic maturation in neural circuits, but the contribution of different neurotransmitter systems is unclear. We investigated the role of GABAA receptor-mediated Cl- conductances in the development of synaptic responses in the Xenopus visual system. Intracellular Cl- concentration ([Cl-]i) was found to be high in immature tectal neurons and then falls over a period of several weeks. GABAergic synapses are present at early stages of tectal development and, when activated by optic nerve stimulation or visual stimuli, induce sustained depolarizing Cl- conductances that facilitate retinotectal transmission by NMDA receptors. To test whether depolarizing GABAergic inputs cooperate with NMDA receptors during activity-dependent maturation of glutamatergic synapses, we prematurely reduced [Cl-]i in tectal neurons in vivo by expressing the Cl- transporter KCC2. This blocked the normal developmental increase in AMPA receptor-mediated retinotectal transmission and increased GABAergic synaptic input to tectal neurons. Therefore, depolarizing GABAergic transmission plays a pivotal role in the maturation of excitatory transmission and controls the balance of excitation and inhibition in the developing retinotectal circuit.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Cell Membrane physiology Chloride Channels physiology Electric Conductivity Excitatory Postsynaptic Potentials physiology Larva Membrane Potentials physiology Neural Inhibition physiology Synaptic Transmission physiology Visual Pathways embryology physiology Xenopus laevis physiology gamma-Aminobutyric Acid metabolism |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor bioinformatics > genomics and proteomics > small molecules > GABAergic Investigative techniques and equipment > electrophysiology organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse organism description > animal behavior > visual organism description > animal > Frog > xenopus |
| CSHL Authors: | |
| Communities: | CSHL labs > Cline lab |
| Depositing User: | CSHL Librarian |
| Date: | 10 May 2006 |
| Date Deposited: | 20 Dec 2011 17:06 |
| Last Modified: | 11 Apr 2018 19:37 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22747 |
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