Zhao, Z. Y., Fang, L., Chen, N. S., Johnsen, R. C., Stein, L., Baillie, D. L. (November 2005) Distinct regulatory elements mediate similar expression patterns in the excretory cell of Caenorhabditis elegans. Journal of Biological Chemistry, 280 (46). pp. 38787-38794. ISSN 0021-9258
Abstract
Identification of cis-regulatory elements and their binding proteins constitutes an important part of understanding gene function and regulation. It is well accepted that co-expressed genes tend to share transcriptional elements. However, recent findings indicate that co-expression data show poor correlation with co-regulation data even in unicellular yeast. This motivates us to experimentally explore whether it is possible that co-expressed genes are subject to differential regulatory control using the excretory cell of Caenorhabditis elegans as an example. Excretory cell is a functional equivalent of human kidney. Transcriptional regulation of gene expression in the cell is largely unknown. We isolated a 10-bp excretory cell-specific cis-element, Ex-1, from a pgp-12 promoter. The significance of the element has been demonstrated by its capacity of converting an intestine-specific promoter into an excretory cell-specific one. We also isolated a cDNA encoding an Ex-1 binding transcription factor, DCP-66, using a yeast one-hybrid screen. Role of the factor in regulation of pgp-12 expression has been demonstrated both in vitro and in vivo. Search for occurrence of Ex-1 reveals that only a small portion of excretory cell-specific promoters contain Ex-1. Two other distinct cis-elements isolated from two different promoters can also dictate the excretory cell-specific expression but are independent of regulation by DCP-66. The results indicate that distinct regulatory elements are able to mediate the similar expression patterns.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Gene Expression C Elegans transcription co-regulation interference co-expression eukaryotes networks |
| Subjects: | organism description > animal > C elegans bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cis-regulatory elements |
| CSHL Authors: | |
| Depositing User: | CSHL Librarian |
| Date: | November 2005 |
| Date Deposited: | 04 Jan 2012 21:05 |
| Last Modified: | 04 Jan 2012 21:05 |
| URI: | https://repository.cshl.edu/id/eprint/22744 |
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