Scheinker, V., Fiore, G., Di Cristo, C., Di Cosmo, A., d'Ischia, M., Enikolopov, G., Palumbo, A. (December 2005) Nitric oxide synthase in the nervous system and ink gland of the cuttlefish Sepia officinalis: molecular cloning and expression. Biochem Biophys Res Commun, 338 (2). pp. 1204-15. ISSN 0006-291X (Print)
Abstract
Nitric oxide (NO) signaling is involved in numerous physiological processes in mollusks, e.g., learning and memory, feeding behavior, neural development, and defence response. We report the first molecular cloning of NOS mRNA from a cephalopod, the cuttlefish Sepia officinalis (SoNOS). SoNOS was cloned using a strategy that involves hybridization of degenerate PCR primers to highly conserved NOS regions, combined with RACE procedure. Two splicing variants of SoNOS, differing by 18 nucleotides, were found in the nervous system and the ink gland of Sepia. In situ hybridization shows that SoNOS is expressed in the immature and mature cells of the ink gland and in the regions of the nervous system that are related to the ink defence system.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Animals Central Nervous System enzymology Cloning Molecular methods Gene Expression Profiling Molecular Sequence Data Nitric Oxide Synthase analysis chemistry metabolism Organ Specificity Sepia enzymology Sequence Homology Amino Acid Tissue Distribution |
| Subjects: | organism description > animal behavior |
| CSHL Authors: | |
| Communities: | CSHL labs > Enikopolov lab |
| Depositing User: | CSHL Librarian |
| Date: | 16 December 2005 |
| Date Deposited: | 05 Jan 2012 16:40 |
| Last Modified: | 01 Mar 2017 15:45 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22695 |
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