Transient and persistent dendritic spines in the neocortex in vivo

Holtmaat, A. J. G. D., Trachtenberg, J. T., Wilbrecht, L., Shepherd, G. M., Zhang, Xi., Knott, G. W., Svoboda, Karel. (January 2005) Transient and persistent dendritic spines in the neocortex in vivo. Neuron, 45 (2). pp. 279-91. ISSN 0896-6273 (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/15664179

DOI: 10.1016/j.neuron.2005.01.003

Abstract

Dendritic spines were imaged over days to months in the apical tufts of neocortical pyramidal neurons (layers 5 and 2/3) in vivo. A fraction of thin spines appeared and disappeared over a few days, while most thick spines persisted for months. In the somatosensory cortex, from postnatal day (PND) 16 to PND 25 spine retractions exceeded additions, resulting in a net loss of spines. The fraction of persistent spines (lifetime > or = 8 days) grew gradually during development and into adulthood (PND 16-25, 35%; PND 35-80, 54%; PND 80-120, 66%; PND 175-225, 73%), providing evidence that synaptic circuits continue to stabilize even in the adult brain, long after the closure of known critical periods. In 6-month-old mice, spines turn over more slowly in visual compared to somatosensory cortex, possibly reflecting differences in the capacity for experience-dependent plasticity in these brain regions.

Item Type:	Paper
Uncontrolled Keywords:	Aging physiology Animals Animals Newborn Cell Differentiation physiology Dendritic Spines physiology ultrastructure Green Fluorescent Proteins Male Mice Mice Inbred C57BL Mice Transgenic Microscopy Fluorescence Neocortex growth & development ultrastructure Neural Pathways/growth & development ultrastructure Neuronal Plasticity physiology Pyramidal Cells physiology ultrastructure Somatosensory Cortex growth & development ultrastructure Synapses physiology ultrastructure Visual Cortex growth & development ultrastructure
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons organs, tissues, organelles, cell types and functions > tissues types and functions > visual cortex
CSHL Authors:	Holtmaat, Anthony Shepherd, Gordon M. G. Svoboda, Karel Trachtenberg, Joshua T. Zhang, Xiaoquin
Communities:	CSHL labs > Svoboda lab
Depositing User:	CSHL Librarian
Date:	20 January 2005
Date Deposited:	12 Jan 2012 20:01
Last Modified:	07 May 2018 15:25
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/22598

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