Harwell, C., Burbach, B., Svoboda, K., Nedivi, E. (October 2005) Regulation of cpg15 expression during single whisker experience in the barrel cortex of adult mice. Journal of Neurobiology, 65 (1). pp. 85-96. ISSN 0022-3034
Abstract
Regulation of gene transcription by neuronal activity is thought to be key to the translation of sensory experience into long-term changes in synaptic structure and function. Here we show that cpgI5, a gene encoding an extracellular signaling molecule that promotes dendritic and axonal growth and synaptic maturation, is regulated in the somatosensory cortex by sensory experience capable of inducing cortical plasticity. Using in situ hybridization, we monitored cpgI5 expression in 4-week-old mouse barrel cortex after trimming all whiskers except D1. We found that cpgI5 expression is depressed in the deprived barrels and enhanced in the barrel column corresponding to the spared D1 whisker. Changes in cpgI5 mRNA levels first appear in layer IV, peak 12 h after deprivation, and then decline rapidly. In layers II/III, changes in cpgI5 expression appear later, peak at 24 h, and persist for days. Induction of cpgI5 expression is significantly diminished in adolescent as well as adult CREB knockout mice. cpgI5's spatio-temporal expression pattern and its regulation by CREB are consistent with a role in experience-dependent plasticity of cortical circuits. Our results suggest that local structural and/or synaptic changes may be a mechanism by which the adult cortex can adapt to peripheral manipulations. (c) 2005 Wiley Periodicals, Inc.
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