Imaging calcium concentration dynamics in small neuronal compartments

Yasuda, R., Nimchinsky, E. A., Scheuss, V., Pologruto, T. A., Oertner, T. G., Sabatini, B. L., Svoboda, K. (February 2004) Imaging calcium concentration dynamics in small neuronal compartments. Sci STKE, 2004 (219). pl5. ISSN 1525-8882 (Electronic)

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DOI: 10.1126/stke.2192004pl5


Calcium and its regulation play central roles diverse physiologic processes. Quantification of calcium concentrations ([Ca2+]) in small neuronal compartments is crucial to understanding Ca2+-dependent signaling. Here, we describe techniques that are optimized for 2-photon imaging of [Ca2+] dynamics in small compartments such as dendrites and dendritic spines.

Item Type: Paper
Uncontrolled Keywords: Animals Calcium Signaling physiology Cell Compartmentation physiology Dendrites chemistry physiology Hippocampus cytology Image Processing Computer-Assisted/instrumentation methods Lasers Microscopy Fluorescence Multiphoton/instrumentation methods Neurons chemistry physiology Patch-Clamp Techniques/instrumentation/methods Rats
Subjects: organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > calcium channel
Investigative techniques and equipment > optical devices > lasers > two-photon excitation laser scanning
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Depositing User: CSHL Librarian
Date: 10 February 2004
Date Deposited: 17 Jan 2012 19:22
Last Modified: 17 Jan 2012 19:22

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