Yasuda, R., Nimchinsky, E. A., Scheuss, V., Pologruto, T. A., Oertner, T. G., Sabatini, B. L., Svoboda, K. (February 2004) Imaging calcium concentration dynamics in small neuronal compartments. Sci STKE, 2004 (219). pl5. ISSN 1525-8882 (Electronic)
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Abstract
Calcium and its regulation play central roles diverse physiologic processes. Quantification of calcium concentrations ([Ca2+]) in small neuronal compartments is crucial to understanding Ca2+-dependent signaling. Here, we describe techniques that are optimized for 2-photon imaging of [Ca2+] dynamics in small compartments such as dendrites and dendritic spines.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Calcium Signaling physiology Cell Compartmentation physiology Dendrites chemistry physiology Hippocampus cytology Image Processing Computer-Assisted/instrumentation methods Lasers Microscopy Fluorescence Multiphoton/instrumentation methods Neurons chemistry physiology Patch-Clamp Techniques/instrumentation/methods Rats |
| Subjects: | organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > calcium channel Investigative techniques and equipment > optical devices > lasers > two-photon excitation laser scanning |
| CSHL Authors: | |
| Depositing User: | CSHL Librarian |
| Date: | 10 February 2004 |
| Date Deposited: | 17 Jan 2012 19:22 |
| Last Modified: | 17 Jan 2012 19:22 |
| URI: | https://repository.cshl.edu/id/eprint/22518 |
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