Hoeppner, D. J., Spector, M. S., Ratliff, T. M., Kinchen, J. M., Granat, S., Lin, S. C., Bhusri, S. S., Conradt, B., Herman, M. A., Hengartner, M. O. (October 2004) eor-1 and eor-2 are required for cell-specific apoptotic death in C. elegans. Dev Biol, 274 (1). pp. 125-38. ISSN 0012-1606 (Print)
Abstract
Programmed cell death occurs in every multicellular organism and in diverse cell types yet the genetic controls that define which cells will live and which will die remain poorly understood. During development of the nematode Caenorhabditis elegans, the coordinated activity of four gene products, EGL-1, CED-9, CED-4 and CED-3, results in the death of essentially all cells fated to die. To identify novel upstream components of the cell death pathway, we performed a genetic screen for mutations that abolish the death of the hermaphrodite-specific neurons (HSNs), a homologous pair of cells required for egg-laying in the hermaphrodite. We identified and cloned the genes, eor-1 and eor-2, which are required to specify the fate of cell death in male HSNs. In addition to defects in HSN death, mutation of either gene leads to defects in coordinated movement, neuronal migration, male tail development, and viability; all consistent with abnormal neuronal differentiation. eor-1 encodes a putative transcription factor related to the human oncogene PLZF. eor-2 encodes a novel but conserved protein. We propose that eor-1 and eor-2 function together throughout the nervous system to promote terminal differentiation of neurons and function specifically in male HSNs to promote apoptotic death of the HSNs.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Animals Apoptosis physiology Base Sequence Caenorhabditis elegans embryology Caenorhabditis elegans Proteins genetics metabolism physiology Cell Differentiation physiology DNA Primers Female Green Fluorescent Proteins Luminescent Proteins metabolism Molecular Sequence Data Mutation genetics Neurons metabolism Nuclear Proteins genetics metabolism physiology Polymerase Chain Reaction Repressor Proteins metabolism Sequence Alignment Sequence Analysis DNA |
| Subjects: | organism description > animal > C elegans organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis |
| CSHL Authors: | |
| Communities: | CSHL labs > Hengartner lab |
| Depositing User: | CSHL Librarian |
| Date: | 1 October 2004 |
| Date Deposited: | 01 Feb 2012 19:12 |
| Last Modified: | 29 Sep 2014 14:59 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22390 |
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