Nuclear Speckles

Spector, D. L., Lamond, A. I. (February 2011) Nuclear Speckles. Cold Spring Harbor Perspectives in Biology, 3 (2). ISSN 1943-0264 (Electronic)

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DOI: 10.1101/cshperspect.a000646


Nuclear speckles, also known as interchromatin granule clusters, are nuclear domains enriched in pre-mRNA splicing factors, located in the interchromatin regions of the nucleoplasm of mammalian cells. When observed by immunofluorescence microscopy, they usually appear as 20-50 irregularly shaped structures that vary in size. Speckles are dynamic structures, and their constituents can exchange continuously with the nucleoplasm and other nuclear locations, including active transcription sites. Studies on the composition, structure, and dynamics of speckles have provided an important paradigm for understanding the functional organization of the nucleus and the dynamics of the gene expression machinery.

Item Type: Paper
Subjects: Investigative techniques and equipment > microscopy > immunoflourescence microscopy
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nuclear speckles
CSHL Authors:
Communities: CSHL Cancer Center Shared Resources > DNA Sequencing Service
CSHL Cancer Center Shared Resources > Microscopy Service
CSHL labs > Spector lab
Depositing User: CSHL Librarian
Date: February 2011
Date Deposited: 19 Oct 2011 13:46
Last Modified: 02 Dec 2016 20:29
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