Astrin regulates Aurora-A localization

Du, J., Jablonski, S., Yen, T. J., Hannon, G. J. (2008) Astrin regulates Aurora-A localization. Biochem Biophys Res Commun, 370 (2). pp. 213-9.


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DOI: 10.1016/j.bbrc.2008.03.072


Alterations in the expression and activity of the centrosomal kinase, Aurora-A/STK15, affect genomic stability, disrupt the fidelity of centrosome duplication, and induce cellular transformation. A mitotic spindle-associated protein, astrin/DEEPEST, was identified as an Aurora-A interacting protein by a two-hybrid screen. Astrin and Aurora-A co-express at mitosis and co-localize to mitotic spindles. RNAi-mediated depletion of astrin abolishes the localization of Aurora-A on mitotic spindles and leads to a moderate mitotic cell cycle delay, which resembles the mitotic arrest phenotypes in siAurora-A treated cells. However, depletion of Aurora-A does not affect astrin localization, and co-depletion of both astrin and Aurora-A causes a mitotic arrest phenotype similar to depletion of siAurora-A alone. These results suggest that astrin acts upstream of Aurora-A to regulate its mitotic spindle localization.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Cell Cycle Proteins/analysis/genetics/*metabolism Centrosome/enzymology Epistasis, Genetic Hela Cells Humans *Mitosis/drug effects/genetics Mitotic Spindle Apparatus/*enzymology Protein-Serine-Threonine Kinases/analysis/genetics/*metabolism RNA Interference Saccharomyces cerevisiae/genetics Two-Hybrid System Techniques
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > telomeres
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Tom Adams
Date: 2008
Date Deposited: 25 Aug 2011 15:38
Last Modified: 02 Dec 2016 16:37
PMCID: PMC2754255
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