Cortical glutamatergic projection neuron types contribute to distinct functional subnetworks

Mohan, Hemanth, An, Xu, Xu, X Hermione, Kondo, Hideki, Zhao, Shengli, Matho, Katherine S, Wang, Bor-Shuen, Musall, Simon, Mitra, Partha, Huang, Z Josh (January 2023) Cortical glutamatergic projection neuron types contribute to distinct functional subnetworks. Nature Neuroscience. ISSN 1097-6256

Abstract

The cellular basis of cerebral cortex functional architecture remains not well understood. A major challenge is to monitor and decipher neural network dynamics across broad cortical areas yet with projection-neuron-type resolution in real time during behavior. Combining genetic targeting and wide-field imaging, we monitored activity dynamics of subcortical-projecting (PTFezf2) and intratelencephalic-projecting (ITPlxnD1) types across dorsal cortex of mice during different brain states and behaviors. ITPlxnD1 and PTFezf2 neurons showed distinct activation patterns during wakeful resting, during spontaneous movements and upon sensory stimulation. Distinct ITPlxnD1 and PTFezf2 subnetworks were dynamically tuned to different sensorimotor components of a naturalistic feeding behavior, and optogenetic inhibition of ITsPlxnD1 and PTsFezf2 in subnetwork nodes disrupted distinct components of this behavior. Lastly, ITPlxnD1 and PTFezf2 projection patterns are consistent with their subnetwork activation patterns. Our results show that, in addition to the concept of columnar organization, dynamic areal and projection-neuron-type specific subnetworks are a key feature of cortical functional architecture linking microcircuit components with global brain networks.

Item Type:	Paper
Subjects:	therapies organism description > animal organs, tissues, organelles, cell types and functions > organs types and functions > brain organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex therapies > deep brain stimulation organs, tissues, organelles, cell types and functions > cell types and functions > cell types > interneurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > interneurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > interneurons organism description > animal > mammal organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions > tissues types and functions > neural networks organs, tissues, organelles, cell types and functions > organs types and functions organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:	Mohan, Hemanth Matho, Katherine Wang, Bor-Shuen Musall, Simon Mitra, Partha P. An, Xu Kondo, Hideki
Communities:	CSHL labs > Churchland lab CSHL labs > Huang lab CSHL labs > Mitra lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	23 January 2023
Date Deposited:	06 Feb 2023 20:45
Last Modified:	10 Jan 2024 20:32
PMCID:	PMC10571488
URI:	https://repository.cshl.edu/id/eprint/40834

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