Chd5 regulates the transcription factor Six3 to promote neuronal differentiation

Shrestha, Padmina, Jaganathan, Anbalagan, Huilgol, Dhananjay, Ballon, Carlos, Hwangbo, Yon, Mills, Alea A (January 2023) Chd5 regulates the transcription factor Six3 to promote neuronal differentiation. Stem Cells. sxad002. ISSN 1066-5099

Abstract

Chromodomain helicase DNA-binding protein 5 (Chd5) is an ATP-dependent chromatin remodeler that promotes neuronal differentiation. However, the mechanism behind the action of Chd5 during neurogenesis is not clearly understood. Here we use transcriptional profiling of cells obtained from Chd5 deficient mice at early and late stages of neuronal differentiation to show that Chd5 regulates neurogenesis by directing stepwise transcriptional changes. During early stages of neurogenesis, Chd5 promotes expression of the proneural transcription factor Six3 to repress Wnt5a, a non-canonical Wnt ligand essential for the maturation of neurons. This previously unappreciated ability of Chd5 to transcriptionally repress neuronal maturation factors is critical for both lineage specification and maturation. Thus, Chd5 facilitates early transcriptional changes in neural stem cells, thereby initiating transcriptional programs essential for neuronal fate specification.

Item Type:	Paper
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > differentiation organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > neurogenesis organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:	Shrestha, Padmina Jaganathan, Anbalagan Huilgol, Dhananjay Ballon, Carlos Mills, Alea A.
Communities:	CSHL labs > Huang lab CSHL labs > Mills lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	13 January 2023
Date Deposited:	23 Jan 2023 20:16
Last Modified:	23 Jan 2023 20:16
URI:	https://repository.cshl.edu/id/eprint/40799

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