A Forward Genetic Approach to Identify Plasmodesmal Trafficking Regulators Based on Trichome Rescue

Kitagawa, Munenori, Jackson, David (March 2022) A Forward Genetic Approach to Identify Plasmodesmal Trafficking Regulators Based on Trichome Rescue. Methods in Molecular Biology, 2457. pp. 393-407. ISSN 1064-3745

URL: https://www.ncbi.nlm.nih.gov/pubmed/35349156
DOI: 10.1007/978-1-0716-2132-5_27


Plasmodesmata (PD) are channels in the walls of plant cells which enable cell-to-cell information transfer. This includes the selective transport of specific transcription factors that control cell fate during plant development. KNOTTED1 (KN1) homeobox (KNOX) family transcription factors that are essential for the maintenance and function of stem cells in shoot meristems use this trafficking pathway, but its mechanism is largely unknown. Here we describe a forward genetic approach to the identification of regulators of selective KN1 trafficking through PD, using a trichome rescue system that permits simple visual analysis in Arabidopsis leaves. A KN1 trafficking regulator identified in this approach had the capacity to regulate the transport not only of KN1 but also of another mobile regulatory protein, TRANSPARENT TESTA GLABRA1 (TTG1). Our system could be easily adapted to reveal the mechanism underlying the selective transport of additional mobile signals through PD.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
organism description > plant behavior > plasmodesmata
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > plasmodesmata
CSHL Authors:
Communities: CSHL labs > Jackson lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 30 March 2022
Date Deposited: 31 Mar 2022 19:56
Last Modified: 31 Mar 2022 19:56
URI: https://repository.cshl.edu/id/eprint/40561

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