Multi-color Flow Cytometry for Comprehensive Analysis of the Tumor Immune Infiltrate in a Murine Model of Breast Cancer.

Almeida, Ana S, Fein, Miriam R, Egeblad, Mikala (June 2021) Multi-color Flow Cytometry for Comprehensive Analysis of the Tumor Immune Infiltrate in a Murine Model of Breast Cancer. Bio-protocol, 11 (11). e4012. ISSN 2331-8325

URL: https://www.ncbi.nlm.nih.gov/pubmed/34250200
DOI: 10.21769/BioProtoc.4012

Abstract

Flow cytometry is a popular laser-based technology that allows the phenotypic and functional characterization of individual cells in a high-throughput manner. Here, we describe a detailed procedure for preparing a single-cell suspension from mammary tumors of the mouse mammary tumor virus-polyoma middle T (MMTV-PyMT) and analyzing these cells by multi-color flow cytometry. This protocol can be used to study the following tumor-infiltrating immune cell populations, defined by the expression of cell surface molecules: total leukocytes, tumor-associated macrophages (TAMs), conventional dendritic cells (DCs), CD103-expressing DCs, tumor-associated neutrophils, inflammatory monocytes, natural killer (NK) cells, CD4+ T cells, CD8+ T cells, γδT cells, and regulatory T cells.

Item Type: Paper
Subjects: diseases & disorders > cancer > cancer types > breast cancer
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > immune cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > immune cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > immune cell
CSHL Authors:
Communities: CSHL labs > Beyaz lab
CSHL labs > Egeblad lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 5 June 2021
Date Deposited: 14 Jul 2021 14:56
Last Modified: 14 Jul 2021 14:56
PMCID: PMC8251661
URI: https://repository.cshl.edu/id/eprint/40292

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