Solution-Phase Exome Capture.

Mardis, Elaine, McCombie, W Richard (August 2017) Solution-Phase Exome Capture. Cold Spring Harbor Protocols, 2017 (8). pdb.prot094680. ISSN 1940-3402

DOI: 10.1101/pdb.prot094680


This protocol describes the construction of a paired-end library of genomic DNA (gDNA) and subsequent capture of specific regions of a genome using NimbleGen sequence capture probes and Illumina TruSeq oligos. The captured DNA, purified and quantitated, is appropriate for use as template in Illumina sequencing systems. A procedure is also provided for magnetic AMPure bead cleanup.

Item Type: Paper
Subjects: Investigative techniques and equipment > whole exome sequencing
Investigative techniques and equipment > assays > whole exome sequencing
CSHL Authors:
Communities: CSHL labs > McCombie lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 1 August 2017
Date Deposited: 24 May 2021 15:37
Last Modified: 24 May 2021 15:37

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