Mardis, Elaine, McCombie, W Richard (August 2017) Solution-Phase Exome Capture. Cold Spring Harbor Protocols, 2017 (8). pdb.prot094680. ISSN 1940-3402
Abstract
This protocol describes the construction of a paired-end library of genomic DNA (gDNA) and subsequent capture of specific regions of a genome using NimbleGen sequence capture probes and Illumina TruSeq oligos. The captured DNA, purified and quantitated, is appropriate for use as template in Illumina sequencing systems. A procedure is also provided for magnetic AMPure bead cleanup.
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment > whole exome sequencing Investigative techniques and equipment > assays > whole exome sequencing |
| CSHL Authors: | |
| Communities: | CSHL labs > McCombie lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 1 August 2017 |
| Date Deposited: | 24 May 2021 15:37 |
| Last Modified: | 24 May 2021 15:37 |
| URI: | https://repository.cshl.edu/id/eprint/40141 |
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