Characterization of Organoid Cultures to Study the Effects of Pregnancy Hormones on the Epigenome and Transcriptional Output of Mammary Epithelial Cells

Ciccone, M. F., Trousdell, M. C., Dos Santos, C. O. (November 2020) Characterization of Organoid Cultures to Study the Effects of Pregnancy Hormones on the Epigenome and Transcriptional Output of Mammary Epithelial Cells. J Mammary Gland Biol Neoplasia, 25 (4). pp. 351-366. ISSN 1083-3021

URL: https://pubmed.ncbi.nlm.nih.gov/33131024/
DOI: 10.1007/s10911-020-09465-0

Abstract

The use of mouse derived mammary organoids can provide a unique strategy to study mammary gland development across a normal life cycle, as well as offering insights into how malignancies form and progress. Substantial cellular and epigenomic changes are triggered in response to pregnancy hormones, a reaction that engages molecular and cellular changes that transform the mammary epithelial cells into "milk producing machines". Such epigenomic alterations remain stable in post-involution mammary epithelial cells and control the reactivation of gene transcription in response to re-exposure to pregnancy hormones. Thus, a system that tightly controls exposure to pregnancy hormones, epigenomic alterations, and activation of transcription will allow for a better understanding of such molecular switches. Here, we describe the characterization of ex vivo cultures to mimic the response of mammary organoid cultures to pregnancy hormones and to understand gene regulation and epigenomic reprogramming on consecutive hormone exposure. Our findings suggest that this system yields similar epigenetic modifications to those reported in vivo, thus representing a suitable model to closely track epigenomic rearrangement and define unknown players of pregnancy-induced development.

Item Type: Paper
Uncontrolled Keywords: Epigenomics Mammary organoids Pregnancy-induced development
Subjects: diseases & disorders > cancer > cancer types > breast cancer
Investigative techniques and equipment > cell culture > cancer organoids
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > epigenetics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > epigenetics
CSHL Authors:
Communities: CSHL labs > Dos Santos lab
Depositing User: Matthew Dunn
Date: 1 November 2020
Date Deposited: 19 Apr 2021 19:45
Last Modified: 29 Jun 2021 15:19
PMCID: PMC7960614
Related URLs:
URI: https://repository.cshl.edu/id/eprint/39852

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