Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy

Ueda, H.R., Dodt, H.-U., Osten, P., Economo, M.N., Chandrashekar, J., Keller, P.J. (May 2020) Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy. Neuron, 106 (3). pp. 369-387. ISSN 0896-6273

URL: https://pubmed.ncbi.nlm.nih.gov/32380050/
DOI: 10.1016/j.neuron.2020.03.004

Abstract

Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields have been combined only recently to facilitate novel experiments and measurements in neuroscience. Since tissue-clearing methods were first combined with modernized light-sheet microscopy a decade ago, the performance of both technologies has rapidly improved, broadening their applications. Here, we review the state of the art of tissue-clearing methods and light-sheet microscopy and discuss applications of these techniques in profiling cells and circuits in mice. We examine outstanding challenges and future opportunities for expanding these techniques to achieve brain-wide profiling of cells and circuits in primates and humans. Such integration will help provide a systems-level understanding of the physiology and pathology of our central nervous system.

Item Type: Paper
CSHL Authors:
Communities: CSHL labs > Osten lab
Depositing User: Adrian Gomez
Date: 6 May 2020
Date Deposited: 11 May 2020 14:28
Last Modified: 06 Jul 2020 15:14
PMCID: PMC7213014
Related URLs:
URI: https://repository.cshl.edu/id/eprint/39355

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving