Ueda, H.R., Dodt, H.-U., Osten, P., Economo, M.N., Chandrashekar, J., Keller, P.J. (May 2020) Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy. Neuron, 106 (3). pp. 369-387. ISSN 0896-6273
Abstract
Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields have been combined only recently to facilitate novel experiments and measurements in neuroscience. Since tissue-clearing methods were first combined with modernized light-sheet microscopy a decade ago, the performance of both technologies has rapidly improved, broadening their applications. Here, we review the state of the art of tissue-clearing methods and light-sheet microscopy and discuss applications of these techniques in profiling cells and circuits in mice. We examine outstanding challenges and future opportunities for expanding these techniques to achieve brain-wide profiling of cells and circuits in primates and humans. Such integration will help provide a systems-level understanding of the physiology and pathology of our central nervous system.
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment organs, tissues, organelles, cell types and functions > organs types and functions > brain Investigative techniques and equipment > imaging organs, tissues, organelles, cell types and functions > organs types and functions organs, tissues, organelles, cell types and functions |
| CSHL Authors: | |
| Communities: | CSHL labs > Osten lab |
| Depositing User: | Adrian Gomez |
| Date: | 6 May 2020 |
| Date Deposited: | 11 May 2020 14:28 |
| Last Modified: | 01 Feb 2024 19:02 |
| PMCID: | PMC7213014 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/39355 |
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