Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation

Haery, L., Deverman, B. E., Matho, K. S., Cetin, A., Woodard, K., Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens, J., Fan, M. (November 2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat, 13. p. 93. ISSN 1662-5129 (Print)1662-5129

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URL: https://www.ncbi.nlm.nih.gov/pubmed/31849618
DOI: 10.3389/fnana.2019.00093

Abstract

Cell-type-specific expression of molecular tools and sensors is critical to construct circuit diagrams and to investigate the activity and function of neurons within the nervous system. Strategies for targeted manipulation include combinations of classical genetic tools such as Cre/loxP and Flp/FRT, use of cis-regulatory elements, targeted knock-in transgenic mice, and gene delivery by AAV and other viral vectors. The combination of these complex technologies with the goal of precise neuronal targeting is a challenge in the lab. This report will discuss the theoretical and practical aspects of combining current technologies and establish best practices for achieving targeted manipulation of specific cell types. Novel applications and tools, as well as areas for development, will be envisioned and discussed.

Item Type: Paper
Additional Information: Frontiers in neuroanatomy
Uncontrolled Keywords: Aav cell-type specificity gene delivery intersectional methods neuroscience targeted neuronal manipulation viral vectors virus technologies
Subjects: organism description > virus > adeno associated virus
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal projections
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal projections
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal projections
CSHL Authors:
Depositing User: Adrian Gomez
Date: 26 November 2019
Date Deposited: 19 Dec 2019 17:52
Last Modified: 19 Dec 2019 17:52
PMCID: PMC6902037
Related URLs:
URI: https://repository.cshl.edu/id/eprint/38806

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