Haery, L., Deverman, B. E., Matho, K. S., Cetin, A., Woodard, K., Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens, J., Fan, M.
(November 2019)
Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation.
Front Neuroanat, 13.
p. 93.
ISSN 1662-5129 (Print)1662-5129
Abstract
Cell-type-specific expression of molecular tools and sensors is critical to construct circuit diagrams and to investigate the activity and function of neurons within the nervous system. Strategies for targeted manipulation include combinations of classical genetic tools such as Cre/loxP and Flp/FRT, use of cis-regulatory elements, targeted knock-in transgenic mice, and gene delivery by AAV and other viral vectors. The combination of these complex technologies with the goal of precise neuronal targeting is a challenge in the lab. This report will discuss the theoretical and practical aspects of combining current technologies and establish best practices for achieving targeted manipulation of specific cell types. Novel applications and tools, as well as areas for development, will be envisioned and discussed.
| Item Type: |
Paper
|
| Additional Information: |
Frontiers in neuroanatomy |
| Uncontrolled Keywords: |
Aav
cell-type specificity
gene delivery
intersectional methods
neuroscience
targeted neuronal manipulation
viral vectors
virus technologies |
| Subjects: |
organism description > virus > adeno associated virus
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal projections
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal projections
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal projections
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions
organism description > virus |
| CSHL Authors: |
|
| Depositing User: |
Adrian Gomez
|
| Date: |
26 November 2019 |
| Date Deposited: |
19 Dec 2019 17:52 |
| Last Modified: |
02 Feb 2024 15:32 |
| PMCID: |
PMC6902037 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/38806 |
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