TrpM8-mediated somatosensation in mouse neocortex

Beukema, P., Cecil, K. L., Peterson, E., Mann, V. R., Matsushita, M., Takashima, Y., Navlakha, S., Barth, A. L. (June 2018) TrpM8-mediated somatosensation in mouse neocortex. J Comp Neurol, 526 (9). pp. 1444-1456. ISSN 0021-9967

URL: https://www.ncbi.nlm.nih.gov/pubmed/29484652
DOI: 10.1002/cne.24418

Abstract

Somatosensation is a complex sense mediated by more than a dozen distinct neural subtypes in the periphery. Although pressure and touch sensation have been mapped to primary somatosensory cortex in rodents, it has been controversial whether pain and temperature inputs are also directed to this area. Here we use a well-defined somatosensory modality, cool sensation mediated by peripheral TrpM8-receptors, to investigate the neural substrate for cool perception in the mouse neocortex. Using activation of cutaneous TrpM8 receptor-expressing neurons, we identify candidate neocortical areas responsive for cool sensation. Initially, we optimized TrpM8 stimulation and determined that menthol, a selective TrpM8 agonist, was more effective than cool stimulation at inducing expression of the immediate-early gene c-fos in the spinal cord. We developed a broad-scale brain survey method for identification of activated brain areas, using automated methods to quantify c-fos immunoreactivity (fos-IR) across animals. Brain areas corresponding to the posterior insular cortex and secondary somatosensory (S2) show elevated fos-IR after menthol stimulation, in contrast to weaker activation in primary somatosensory cortex (S1). In addition, menthol exposure triggered fos-IR in piriform cortex, the amygdala, and the hypothalamus. Menthol-mediated activation was absent in TrpM8-knock-out animals. Our results indicate that cool somatosensory input broadly drives neural activity across the mouse brain, with neocortical signal most elevated in the posterior insula, as well as S2 and S1. These findings are consistent with data from humans indicating that the posterior insula is specialized for somatosensory information encoding temperature, pain, and gentle touch.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > immediate early genes
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > tissues types and functions > somatosensory cortex
CSHL Authors:
Communities: CSHL labs > Navlakha lab
Depositing User: Matthew Dunn
Date: 15 June 2018
Date Deposited: 06 Nov 2019 15:20
Last Modified: 06 Nov 2019 15:22
PMCID: PMC5899639
Related URLs:
URI: https://repository.cshl.edu/id/eprint/38631

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