Regulators of cell movement during development and regeneration in Drosophila

Myat, M. M., Louis, D., Mavrommatis, A., Collins, L., Mattis, J., Ledru, M., Verghese, S., Su, T. T. (May 2019) Regulators of cell movement during development and regeneration in Drosophila. Open Biol, 9 (5). p. 180245. ISSN 2046-2441

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DOI: 10.1098/rsob.180245


Cell migration is a fundamental cell biological process essential both for normal development and for tissue regeneration after damage. Cells can migrate individually or as a collective. To better understand the genetic requirements for collective migration, we expressed RNA interference (RNAi) against 30 genes in the Drosophila embryonic salivary gland cells that are known to migrate collectively. The genes were selected based on their effect on cell and membrane morphology, cytoskeleton and cell adhesion in cell culture-based screens or in Drosophila tissues other than salivary glands. Of these, eight disrupted salivary gland migration, targeting: Rac2, Rab35 and Rab40 GTPases, MAP kinase-activated kinase-2 (MAPk-AK2), RdgA diacylglycerol kinase, Cdk9, the PDSW subunit of NADH dehydrogenase (ND-PDSW) and actin regulator Enabled (Ena). The same RNAi lines were used to determine their effect during regeneration of X-ray-damaged larval wing discs. Cells translocate during this process, but it remained unknown whether they do so by directed cell divisions, by cell migration or both. We found that RNAi targeting Rac2, MAPk-AK2 and RdgA disrupted cell translocation during wing disc regeneration, but RNAi against Ena and ND-PDSW had little effect. We conclude that, in Drosophila, cell movements in development and regeneration have common as well as distinct genetic requirements.

Item Type: Paper
Subjects: organism description > animal > insect > Drosophila
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell migration
organs, tissues, organelles, cell types and functions > tissues types and functions > Salivary Glands
CSHL Authors:
Communities: School of Biological Sciences > SBS Administration
Depositing User: Matthew Dunn
Date: 31 May 2019
Date Deposited: 29 Jul 2019 13:34
Last Modified: 09 Sep 2021 18:28
PMCID: PMC6544984
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