Genetic Single Neuron Anatomy Reveals Fine Granularity of Cortical Axo-Axonic Cells

Wang, X., Tucciarone, J., Jiang, S., Yin, F., Wang, B. S., Wang, D., Jia, Y., Jia, X., Li, Y., Yang, T., Xu, Z., Akram, M. A., Wang, Y., Zeng, S., Ascoli, G. A., Mitra, P., Gong, H., Luo, Q., Huang, Z. J. (March 2019) Genetic Single Neuron Anatomy Reveals Fine Granularity of Cortical Axo-Axonic Cells. Cell Rep, 26 (11). 3145-3159.e5.

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Abstract

Parsing diverse nerve cells into biological types is necessary for understanding neural circuit organization. Morphology is an intuitive criterion for neuronal classification and a proxy of connectivity, but morphological diversity and variability often preclude resolving the granularity of neuron types. Combining genetic labeling with high-resolution, large-volume light microscopy, we established a single neuron anatomy platform that resolves, registers, and quantifies complete neuron morphologies in the mouse brain. We discovered that cortical axo-axonic cells (AACs), a cardinal GABAergic interneuron type that controls pyramidal neuron (PyN) spiking at axon initial segments, consist of multiple subtypes distinguished by highly laminar-specific soma position and dendritic and axonal arborization patterns. Whereas the laminar arrangements of AAC dendrites reflect differential recruitment by input streams, the laminar distribution and local geometry of AAC axons enable differential innervation of PyN ensembles. This platform will facilitate genetically targeted, high-resolution, and scalable single neuron anatomy in the mouse brain.

Item Type:	Paper
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
CSHL Authors:	Mitra, Partha P. Huang, Z. Josh
Communities:	CSHL labs > Mitra lab CSHL labs > Huang lab
Depositing User:	Matthew Dunn
Date:	12 March 2019
Date Deposited:	25 Mar 2019 19:42
Last Modified:	17 Apr 2020 14:56
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/37762

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