Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM

Tai, Y., Gallo, N. B., Wang, M., Yu, J. R., Van Aelst, L. (February 2019) Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM. Neuron, 102 (2). pp. 267-270. ISSN 0896-6273

URL: https://www.ncbi.nlm.nih.gov/pubmed/30846310
DOI: 10.1016/j.neuron.2019.02.009

Abstract

Among the diverse interneuron subtypes in the neocortex, chandelier cells (ChCs) are the only population that selectively innervate pyramidal neurons (PyNs) at their axon initial segment (AIS), the site of action potential initiation, allowing them to exert powerful control over PyN output. Yet, mechanisms underlying their subcellular innervation of PyN AISs are unknown. To identify molecular determinants of ChC/PyN AIS innervation, we performed an in vivo RNAi screen of PyN-expressed axonal cell adhesion molecules (CAMs) and select Ephs/ephrins. Strikingly, we found the L1 family member L1CAM to be the only molecule required for ChC/PyN AIS innervation. Further, we show that L1CAM is required during both the establishment and maintenance of innervation, and that selective innervation of PyN AISs by ChCs requires AIS anchoring of L1CAM by the cytoskeletal ankyrin-G/betaIV-spectrin complex. Thus, our findings identify PyN-expressed L1CAM as a critical CAM required for innervation of neocortical PyN AISs by ChCs.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neocortical interneurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neocortical interneurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neocortical interneurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neocortical interneurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neocortical interneurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neocortical interneurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
CSHL Authors:
Communities: CSHL labs > Van Aelst lab
Depositing User: Matthew Dunn
Date: 21 February 2019
Date Deposited: 12 Mar 2019 19:51
Last Modified: 20 Nov 2019 20:12
PMCID: PMC6525570
Related URLs:
URI: https://repository.cshl.edu/id/eprint/37729

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