Enzymatic in vitro synthesis of globin genes

Efstratiadis, A., Kafatos, F. C., Maxam, A. M., Maniatis, T. (February 1976) Enzymatic in vitro synthesis of globin genes. Cell, 7 (2). pp. 279-88.

URL: http://www.ncbi.nlm.nih.gov/PubMed/60178


Full-length, single-stranded rabbit globin cDNA, synthesized by AMV reverse transcriptase, apparently contains a small double-stranded sequence (hairpin) at the 3' terminus. This cDNA can serve as template-primer for E. coli DNA polymerase I, which synthesizes a strand complementary to the cDNA and covalently bound to it.

Item Type: Paper
Subjects: organism description > bacteria > escherichia coli
organism description > animal > mammal
Communities: Cold Spring Harbor Laboratory of Quantitative Biology
Depositing User: Elizabeth Pessala
Date: February 1976
Date Deposited: 25 Feb 2019 16:25
Last Modified: 25 Feb 2019 16:25
URI: https://repository.cshl.edu/id/eprint/37555

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving