Next-Generation Sequencing Technologies

McCombie, W. R., McPherson, J. D., Mardis, E. R. (November 2018) Next-Generation Sequencing Technologies. Cold Spring Harb Perspect Med, 9 (11). ISSN 2157-1422

DOI: 10.1101/cshperspect.a036798


Although DNA and RNA sequencing has a history spanning five decades, large-scale massively parallel sequencing, or next-generation sequencing (NGS), has only been commercially available for about 10 years. Nonetheless, the meteoric increase in sequencing throughput with NGS has dramatically changed our understanding of our genome and ourselves. Sequencing the first human genome as a haploid reference took nearly 10 years but now a full diploid human genome sequence can be accomplished in just a few days. NGS has also reduced the cost of generating sequence data and a plethora of sequence-based methods for probing a genome have emerged using NGS as the readout and have been applied to many species. NGS methods have also entered the medical realm and will see an increasing use in diagnosis and treatment. NGS has largely been driven by short-read generation (150 bp) but new platforms have emerged and are now capable of generating long multikilobase reads. These latter platforms enable reference-independent genome assemblies and long-range haplotype generation. Rapid DNA and RNA sequencing is now mainstream and will continue to have an increasing impact on biology and medicine.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes > comparative genomics
Investigative techniques and equipment > assays > next generation sequencing
CSHL Authors:
Communities: CSHL labs > McCombie lab
CSHL Cancer Center Program > Cancer Genetics and Genomics Program
Depositing User: Matthew Dunn
Date: 26 November 2018
Date Deposited: 20 Dec 2018 16:21
Last Modified: 14 Dec 2020 16:38
PMCID: PMC6824406
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