Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI

Pettersson, U., Mulder, C., Deluis, H., Sharp, P. A. (January 1973) Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A, 70 (1). pp. 200-4.

URL: http://www.pubmedcentral.nih.gov/4567334

Abstract

The DNA of adenovirus type 2 was cleaved by restriction endonuclease R·RI into six fragments. These fragments were separated by electrophoresis on composite agarose-polyacrylamide gels. Their molecular weights ranged from 1.1 × 106 to 13.6 × 106, as measured by electron microscopy. Each fragment represented a unique segment of adenovirus type 2 DNA since: (i) the fragments were obtained in equimolar amounts; (ii) the sum of their molecular weights was equal to the molecular weight of complete adenovirus DNA; and (iii) each fragment exhibited a rate of renaturation that was inversely proportional to its size.

Item Type: Paper
Subjects: organism description > virus > adenovirus
CSHL Authors:
Communities: CSHL labs
Depositing User: Elizabeth Pessala
Date: January 1973
Date Deposited: 11 Dec 2018 15:42
Last Modified: 11 Dec 2018 15:42
PMCID: PMC433215
URI: https://repository.cshl.edu/id/eprint/37397

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