Testing Susceptibility of Patient-Derived Organoid Cultures to Therapies: Pharmacotyping

Burkhart, R. A., Baker, L. A., Tiriac, H. (May 2018) Testing Susceptibility of Patient-Derived Organoid Cultures to Therapies: Pharmacotyping. Methods Mol Biol, 1787. pp. 253-261. ISSN 1064-3745

Abstract

Increasingly, patient models of disease are being utilized to facilitate precision medicine approaches through molecular characterization or direct chemotherapeutic testing. Organoids, 3-dimensional (3D) cultures of neoplastic cells derived from primary tumor specimens, represent an ideal platform for these types of studies because benchtop protocols previously developed for 2-dimensional cell lines can be adapted for use. These protocols include directly testing the survival of these organoid cultures when exposed to clinically relevant chemotherapeutic agents, a process we have called pharmacotyping. In this protocol, established tumor-derived organoid cultures are dissociated into single cells, plated in a 3D gel matrix, and exposed to pharmacologic agents. While our protocol has been developed for use with patient-derived pancreatic ductal adenocarcinoma organoids, with minor modifications to the dissociation and medium conditions, this protocol could be adapted for use with a wide range of organoid cultures. We further describe our standard ATP-based assay to determine cellular survival. This protocol can be scaled for use in high-throughput assays.

Item Type:	Paper
Uncontrolled Keywords:	3D culture Drug testing Organoids Pharmacotyping Tumor models
Subjects:	diseases & disorders > cancer diseases & disorders Investigative techniques and equipment Investigative techniques and equipment > cell culture > cancer organoids Investigative techniques and equipment > cell culture organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions diseases & disorders > cancer > drugs and therapies organs, tissues, organelles, cell types and functions
CSHL Authors:	Baker, Lindsey Tiriac, Herve Burkhart, Richard
Communities:	CSHL labs > Tuveson lab
Depositing User:	Matt Covey
Date:	May 2018
Date Deposited:	14 Jun 2018 21:20
Last Modified:	07 Feb 2024 18:38
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/36723

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