Task Learning Promotes Plasticity of Interneuron Connectivity Maps in the Olfactory Bulb

Huang, L., Ung, K., Garcia, I., Quast, K. B., Cordiner, K., Saggau, P., Arenkiel, B. R. (August 2016) Task Learning Promotes Plasticity of Interneuron Connectivity Maps in the Olfactory Bulb. Journal of Neuroscience, 36 (34). pp. 8856-8871. ISSN 1529-2401

URL: http://www.ncbi.nlm.nih.gov/pubmed/27559168
DOI: 10.1523/JNEUROSCI.0794-16.2016


Elucidating patterns of functional synaptic connectivity and deciphering mechanisms of how plasticity influences such connectivity is essential toward understanding brain function. In the mouse olfactory bulb (OB), principal neurons (mitral/tufted cells) make reciprocal connections with local inhibitory interneurons, including granule cells (GCs) and external plexiform layer (EPL) interneurons. Our current understanding of the functional connectivity between these cell types, as well as their experience-dependent plasticity, remains incomplete. By combining acousto-optic deflector-based scanning microscopy and genetically targeted expression of Channelrhodopsin-2, we mapped connections in a cell-type-specific manner between mitral cells (MCs) and GCs or between MCs and EPL interneurons. We found that EPL interneurons form broad patterns of connectivity with MCs, whereas GCs make more restricted connections with MCs. Using an olfactory associative learning paradigm, we found that these circuits displayed differential features of experience-dependent plasticity. Whereas reciprocal connectivity between MCs and EPL interneurons was nonplastic, the connections between GCs and MCs were dynamic and adaptive. Interestingly, experience-dependent plasticity of GCs occurred only in certain stages of neuronal maturation. We show that different interneuron subtypes form distinct connectivity maps and modes of experience-dependent plasticity in the OB, which may reflect their unique functional roles in information processing. © 2016 the authors.

Item Type: Paper
Uncontrolled Keywords: Circuit Interneuron Mitral Olfactory Optogenetics Plasticity
Subjects: organism description > animal behavior > learning
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > neural plasticity
organs, tissues, organelles, cell types and functions > tissues types and functions > olfactory bulb
CSHL Authors:
Communities: CSHL labs > Zador lab
Depositing User: Matt Covey
Date: 24 August 2016
Date Deposited: 08 Sep 2016 20:22
Last Modified: 08 Jul 2021 14:52
PMCID: PMC4995301
Related URLs:
URI: https://repository.cshl.edu/id/eprint/33436

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