Immunofluorescent and ultrastructural studies of "sarcomeric" units in stress fibers of cultured non-muscle cells

Gordon, W. E. (December 1978) Immunofluorescent and ultrastructural studies of "sarcomeric" units in stress fibers of cultured non-muscle cells. Exp Cell Res, 117 (2). pp. 253-60. ISSN 0014-4827 (Print)0014-4827 (Linking)

DOI: 10.1016/0014-4827(78)90138-6


Immunofluorescence and electron microscopy were used to study the organization of actin, myosin and α-actinin in the “sarcomeric” units of the stress fibers of a selected non-muscle cell type. The results of indirect immunofluorescence confirm that myosin and α-actinin are periodically distributed in discrete units along stress fibers and demonstrate that they are alternately spaced. This relationship is required by a sarcomere model of stress fiber construction. A comparison between immunofluorescent and EM images of stress fibers confirms that α-actinin is confined to Z line-like dense bodies, myosin to the spaces in between. The most intriguing result is that by immunofluorescence a periodic distribution of actin can be detected in some fibers. This may indicate that even actin is periodically distributed in non-muscle “sarcomeres”.

Item Type: Paper
Uncontrolled Keywords: Actinin/analysis Actins/analysis Cell Line Contractile Proteins/*analysis Cytoplasm/*ultrastructure Cytoskeleton/analysis/*ultrastructure Fluorescent Antibody Technique Myosins/analysis
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > actin
Investigative techniques and equipment > microscopy > immunoflourescence microscopy
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: December 1978
Date Deposited: 25 Aug 2016 12:35
Last Modified: 25 Aug 2016 12:35
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