Direct observation of kinesin stepping by optical trapping interferometry

Svoboda, Karel, Schmidt, Christoph F., Schnapp, Bruce J., Block, Steven M. (October 1993) Direct observation of kinesin stepping by optical trapping interferometry. Nature, 365 (6448). pp. 721-7. ISSN 0028-0836

DOI: 10.1038/365721a0


Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.

Item Type: Paper
Uncontrolled Keywords: Adenosine Triphosphate/physiology Animals Cattle Decapodiformes Interferometry Kinesin/*physiology Lasers Light Microspheres Microtubules/physiology Movement/physiology Silicon Dioxide
Subjects: Investigative techniques and equipment > microscopy > electron microscopy
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > kinesin-5 protein
Investigative techniques and equipment > microscopy
Investigative techniques and equipment > optical devices > microscope techniques or equipment
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > microtubule
CSHL Authors:
Communities: CSHL labs > Svoboda lab
Depositing User: Matt Covey
Date: 21 October 1993
Date Deposited: 12 Apr 2016 15:47
Last Modified: 12 Apr 2016 15:47
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