Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius

Rosenfeld, J. A., Reeves, D., Brugler, M. R., Narechania, A., Simon, S., Durrett, R., Foox, J., Shianna, K., Schatz, M. C., Gandara, J., Afshinnekoo, E., Lam, E. T., Hastie, A. R., Chan, S., Cao, H., Saghbini, M., Kentsis, A., Planet, P. J., Kholodovych, V., Tessler, M., Baker, R., DeSalle, R., Sorkin, L. N., Kolokotronis, S. O., Siddall, M. E., Amato, G., Mason, C. E. (February 2016) Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius. Nat Commun, 7. p. 10164. ISSN 2041-1723 (Electronic)2041-1723 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/26836631
DOI: 10.1038/ncomms10164

Abstract

The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971 kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding and noncoding gene models. The most pronounced change in gene expression during the life cycle occurs after feeding on human blood and included genes from the Wolbachia endosymbiont, which shows a simultaneous and coordinated host/commensal response to haematophagous activity. These data provide a rich genetic resource for mapping activity and density of C. lectularius across human hosts and cities, which can help track, manage and control bed bug infestations.

Item Type: Paper
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
Investigative techniques and equipment > assays > next generation sequencing
Investigative techniques and equipment > assays > RNA-seq
CSHL Authors:
Communities: CSHL labs > Schatz lab
Depositing User: Matt Covey
Date: 2 February 2016
Date Deposited: 09 Feb 2016 20:27
Last Modified: 10 Sep 2019 18:11
Related URLs:
URI: https://repository.cshl.edu/id/eprint/32359

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