Hypotension in transgenic mice expressing atrial natriuretic factor fusion genes

Steinhelper, M. E., Cochrane, K. L., Field, L. J. (September 1990) Hypotension in transgenic mice expressing atrial natriuretic factor fusion genes. Hypertension, 16 (3). pp. 301-7. ISSN 0194-911X (Print)0194-911X (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2144261
DOI: 10.1161/01.HYP.16.3.301


Chronic regulation of the cardiovascular system by atrial natriuretic factor was investigated by generating transgenic mice with elevated hormone levels in the systemic circulation. A fusion gene comprising the mouse transthyretin promoter and mouse atrial natriuretic factor structural sequences was designed so as to target hormone expression to the liver. Hepatic expression of atrial natriuretic factor was detectable as early as embryonic day 15 in transgenic animals. In adult transgenic mice, plasma immunoreactive atrial natriuretic factor concentration was elevated at least eightfold as compared with nontransgenic littermates. The mean arterial pressure of conscious transgenic mice was 75.5 +/- 0.9 mm Hg, significantly less than that of nontransgenic siblings (103.9 +/- 2.0 mm Hg). This difference in mean arterial pressure was not accompanied by significant changes in several other physiological parameters, including heart rate, plasma and urinary electrolytes, water intake, and urine volume. This study demonstrates that a chronic elevation of plasma atrial natriuretic factor decreases arterial blood pressure without inducing diuresis and natriuresis in transgenic mice and also illustrates the value of the transgenic approach for the study of the cardiovascular system.

Item Type: Paper
Uncontrolled Keywords: Animals Atrial Natriuretic Factor/blood/*genetics Blood Pressure Cloning, Molecular Gene Expression Hypotension/*etiology Mice Mice, Inbred C3H Mice, Transgenic/*physiology Prealbumin/*genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs
CSHL labs > Stillman lab
Depositing User: Matt Covey
Date: September 1990
Date Deposited: 29 Mar 2016 14:28
Last Modified: 29 Mar 2016 14:28
Related URLs:
URI: https://repository.cshl.edu/id/eprint/32296

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