Expression of constitutively active CaMKII in target tissue modifies presynaptic axon arbor growth

Zou, D. J., Cline, H. T. (March 1996) Expression of constitutively active CaMKII in target tissue modifies presynaptic axon arbor growth. Neuron, 16 (3). pp. 529-39. ISSN 0896-6273 (Print)

Abstract

Calcium/calmodulin-dependent protein kinase II (CaMKII) can be regulated by synaptic activity and could therefore be involved in activity-dependent control of neuronal growth. We tested whether increased CaMKII activity in postsynaptic optic tectal neurons can modify the development of retinotectal axons in Xenopus. The elaboration of individual presynaptic retinal axons was observed in vivo before and up to 3 days after infecting the tectal cells with vaccinia virus carrying the gene for constitutively active truncated CaMKII (tCaMKII). Elevated postsynaptic CaMKII activity prevented the axons from developing the complexity of normal arbors by increasing the normal rate of branch retractions. Some effects of tCaMKII on arbor morphology were seen 1 day after infection, but they became more dramatic by the third day. The results suggest that postsynaptic CaMKII plays a role in the development of presynaptic arbor structure.

Item Type:	Paper
Uncontrolled Keywords:	Animals Axons/ physiology Calcium/ metabolism Calmodulin/ metabolism Cell Division/ drug effects Protein Kinases/ metabolism/ pharmacology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Time Factors Xenopus laevis
Subjects:	organs, tissues, organelles, cell types and functions > tissues types and functions > axon > axon arbors bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > CaMKII organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > Protein kinase C organism description > animal > Frog > xenopus
CSHL Authors:	Cline, Hollis T. Zou, Dong-Jing
Communities:	CSHL labs > Cline lab
Depositing User:	Kathleen Darby
Date:	March 1996
Date Deposited:	12 May 2014 17:25
Last Modified:	12 May 2014 17:25
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/30129

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