p47 is a cofactor for p97-mediated membrane fusion

Kondo, H., Rabouille, C., Newman, R., Levine, T. P., Pappin, D., Freemont, P., Warren, G. (July 1997) p47 is a cofactor for p97-mediated membrane fusion. Nature, 388 (6637). pp. 75-8. ISSN 0028-0836 (Print)0028-0836 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9214505
DOI: 10.1038/40411


At least two distinct ATPases, NSF and p97, are known to be involved in the heterotypic fusion of transport vesicles with their target membranes and the homotypic fusion of membrane compartments. The NSF-mediated fusion pathway is the best characterized, many of the components having been identified and their functions analysed. In contrast, none of the accessory proteins for the p97-mediated fusion pathway has been identified. Now we have identified the first such component, a protein of relative molecular mass 47,000 (p47), which forms a tight, stoichiometric complex with cytosolic p97 (one trimer of p47 per hexamer of p97). It is essential for the p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments, a process restricted to animal cells. As a homologue of p47 exists in budding yeast, this indicates that it might also be involved in other membrane fusion reactions catalysed by p97, such as karyogamy.

Item Type: Paper
Additional Information: Kondo, H Rabouille, C Newman, R Levine, T P Pappin, D Freemont, P Warren, G England Nature Nature. 1997 Jul 3;388(6637):75-8.
Uncontrolled Keywords: Adenosine Triphosphatases/chemistry/genetics/isolation & purification/ physiology Amino Acid Sequence Animals Carrier Proteins/chemistry/genetics/isolation & purification/ physiology Cloning, Molecular Coenzymes/chemistry/physiology Fungal Proteins/chemistry Golgi Apparatus/physiology HeLa Cells Humans Intracellular Signaling Peptides and Proteins Membrane Fusion/ physiology Membrane Proteins/chemistry/genetics/isolation & purification/ physiology Molecular Sequence Data N-Ethylmaleimide-Sensitive Proteins Rats Recombinant Proteins/chemistry/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Vesicular Transport Proteins
Subjects: organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > ATPase
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning
organs, tissues, organelles, cell types and functions > organelles, types and functions > golgi
organs, tissues, organelles, cell types and functions > tissues types and functions > membranes
CSHL Authors:
Communities: CSHL labs > Pappin lab
Depositing User: Kathleen Darby
Date: 3 July 1997
Date Deposited: 08 May 2014 14:21
Last Modified: 08 May 2014 14:21
Related URLs:
URI: https://repository.cshl.edu/id/eprint/30000

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