In vivo dendritic calcium dynamics in deep-layer cortical pyramidal neurons

Helmchen, F., Svoboda, K., Denk, W., Tank, D. W. (November 1999) In vivo dendritic calcium dynamics in deep-layer cortical pyramidal neurons. Nature Neuroscience, 2 (11). pp. 989-96. ISSN 1097-6256

DOI: 10.1038/14788


Dendritic Ca2+ action potentials in neocortical pyramidal neurons have been characterized in brain slices, but their presence and role in the intact neocortex remain unclear. Here we used two-photon microscopy to demonstrate Ca2+ electrogenesis in apical dendrites of deep-layer pyramidal neurons of rat barrel cortex in vivo. During whisker stimulation, complex spikes recorded intracellularly from distal dendrites and sharp waves in the electrocorticogram were accompanied by large dendritic [Ca2+ ] transients; these also occurred during bursts of action potentials recorded from somata of identified layer 5 neurons. The amplitude of the [Ca 2+] transients was largest proximal to the main bifurcation, where sodium action potentials produced little Ca2+ influx. In some cases, synaptic stimulation evoked [Ca2+] transients without a concomitant action potential burst, suggesting variable coupling between dendrite and soma.

Item Type: Paper
Uncontrolled Keywords: Action Potentials/physiology Animals Calcium/*metabolism Cerebral Cortex/*metabolism Dendrites/*metabolism Pyramidal Cells/*metabolism Rats Rats, Sprague-Dawley
Subjects: organs, tissues, organelles, cell types and functions > organs types and functions > metabolism
organs, tissues, organelles, cell types and functions
CSHL Authors:
Communities: CSHL labs > Svoboda lab
Depositing User: Kathleen Darby
Date: November 1999
Date Deposited: 22 Apr 2014 17:39
Last Modified: 22 Apr 2014 17:39
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