Maletic-Savatic, M., Malinow, R., Svoboda, K. (March 1999) Rapid dendritic morphogenesis in CA1 hippocampal dendrites induced by synaptic activity. Science, 283 (5409). pp. 1923-7. ISSN 0036-8075 (Print)
Abstract
Activity shapes the structure of neurons and their circuits. Two-photon imaging of CA1 neurons expressing enhanced green fluorescent protein in developing hippocampal slices from rat brains was used to characterize dendritic morphogenesis in response to synaptic activity. High-frequency focal synaptic stimulation induced a period (longer than 30 minutes) of enhanced growth of small filopodia-like protrusions (typically less than 5 micrometers long). Synaptically evoked growth was long-lasting and localized to dendritic regions close (less than 50 micrometers) to the stimulating electrode and was prevented by blockade of N-methyl-D-aspartate receptors. Thus, synaptic activation can produce rapid input-specific changes in dendritic structure. Such persistent structural changes could contribute to the development of neural circuitry.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | 2-Amino-5-phosphonovalerate/pharmacology Animals Culture Techniques Dendrites/drug effects/physiology/ ultrastructure Electric Stimulation Excitatory Amino Acid Antagonists/pharmacology Green Fluorescent Proteins Hippocampus/cytology Luminescent Proteins/genetics Microscopy/methods Morphogenesis/drug effects Pseudopodia/drug effects/physiology/ ultrastructure Pyramidal Cells/physiology/ ultrastructure/virology Rats Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors/ physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Sindbis Virus/genetics/physiology Synapses/ physiology/ultrastructure Synaptic Membranes/physiology Synaptic Transmission |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus Investigative techniques and equipment > microscopy organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse |
| CSHL Authors: | |
| Communities: | CSHL labs > Enikopolov lab CSHL labs > Malinow lab CSHL labs > Svoboda lab |
| Depositing User: | Kathleen Darby |
| Date: | 19 March 1999 |
| Date Deposited: | 30 Apr 2014 13:47 |
| Last Modified: | 30 Apr 2014 13:47 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29802 |
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