Rapid Cap: A new generation of mouse models for prostate cancer

Cho, H. J., Herzka, T., Zheng, W., Castillo-Martin, M., Cordon-Cardo, C., Trotman, L. C. (April 2013) Rapid Cap: A new generation of mouse models for prostate cancer. Cancer Research, 73 (8 (Sup). p. 4091. ISSN 0008-5472

URL: http://cancerres.aacrjournals.org/cgi/content/shor...
DOI: 10.1158/1538-7445.am2013-4091


Genetically Engineered Mouse Models are the gold standard for functional cancer research. However, the associated time and cost requirements severely limit their application. As a consequence, projects carry typically a high risk, are lengthy, and scientists become ‘locked in’ with a few chosen candidate alterations. To realize the full potential of mouse modeling technology we have developed RapidCaP, a system that relies on surgical gene transfer. Through prostate specific delivery of transgenic virus we can 1) reduce model generation times from several years to a few weeks, 2) test various genetic alterations such as loss or gain of function, alone or in combination, and 3) use non-invasive imaging to monitor disease progression. Using RapidCaP, we show that focal loss of Pten and Trp53 genes triggers prostate lesions within 2 months. Furthermore, we find that this disease responds to castration by strong regression within several weeks, but it later relapses to produce lethal hormone refractory disease. Taken together, our approach establishes a novel platform for basic prostate cancer research and it realizes the goal of carrying out pre-clinical studies in genetically engineered mice.

Item Type: Paper
Additional Information: Meeting Abstract
Subjects: diseases & disorders > cancer
Publication Type > Meeting Abstract
organism description > model organism
organism description > animal > mammal > rodent > mouse
diseases & disorders > cancer > cancer types > prostate cancer
CSHL Authors:
Communities: CSHL labs > Trotman lab
Depositing User: Matt Covey
Date: April 2013
Date Deposited: 11 Apr 2014 16:43
Last Modified: 21 Feb 2018 15:55
URI: https://repository.cshl.edu/id/eprint/29754

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