Cho, H. J., Herzka, T., Zheng, W., Castillo-Martin, M., Cordon-Cardo, C., Trotman, L. C. (April 2013) Rapid Cap: A new generation of mouse models for prostate cancer. Cancer Research, 73 (8 (Sup). p. 4091. ISSN 0008-5472
Abstract
Genetically Engineered Mouse Models are the gold standard for functional cancer research. However, the associated time and cost requirements severely limit their application. As a consequence, projects carry typically a high risk, are lengthy, and scientists become ‘locked in’ with a few chosen candidate alterations. To realize the full potential of mouse modeling technology we have developed RapidCaP, a system that relies on surgical gene transfer. Through prostate specific delivery of transgenic virus we can 1) reduce model generation times from several years to a few weeks, 2) test various genetic alterations such as loss or gain of function, alone or in combination, and 3) use non-invasive imaging to monitor disease progression. Using RapidCaP, we show that focal loss of Pten and Trp53 genes triggers prostate lesions within 2 months. Furthermore, we find that this disease responds to castration by strong regression within several weeks, but it later relapses to produce lethal hormone refractory disease. Taken together, our approach establishes a novel platform for basic prostate cancer research and it realizes the goal of carrying out pre-clinical studies in genetically engineered mice.
| Item Type: | Paper |
|---|---|
| Additional Information: | Meeting Abstract |
| Subjects: | diseases & disorders > cancer Publication Type > Meeting Abstract organism description > model organism organism description > animal > mammal > rodent > mouse diseases & disorders > cancer > cancer types > prostate cancer |
| CSHL Authors: | |
| Communities: | CSHL labs > Trotman lab |
| Depositing User: | Matt Covey |
| Date: | April 2013 |
| Date Deposited: | 11 Apr 2014 16:43 |
| Last Modified: | 21 Feb 2018 15:55 |
| URI: | https://repository.cshl.edu/id/eprint/29754 |
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