atSRp30, one of two SF2/ASF-like proteins from Arabidopsis thaliana, regulates splicing of specific plant genes

Lopato, S., Kalyna, M., Dorner, S., Kobayashi, R., Krainer, A. R., Barta, A. (April 1999) atSRp30, one of two SF2/ASF-like proteins from Arabidopsis thaliana, regulates splicing of specific plant genes. Genes & Development, 13 (8). pp. 987-1001. ISSN 0890-9369

Preview

PDF (Paper) Krainer and Kobayashi Genes and Development 1999b.pdf - Published Version Download (3MB) | Preview

Abstract

SR proteins are nuclear phosphoproteins with a characteristic Ser/Arg-rich domain and one or two RNA recognition motifs. They are highly conserved in animals and plants and play important roles in spliceosome assembly and alternative splicing regulation. We have now isolated and partially sequenced a plant protein, which crossreacts with antibodies to human SR proteins. The sequence of the corresponding cDNA and genomic clones from Arabidopsis revealed a protein, atSRp3a, with strong similarity to the human SR protein SF2/ASF and to atSRp34/SR1, a previously identified SR protein, indicating that plants possess two SP2/ASF-like proteins, atSRp30 expresses alternatively spliced mRNA isoforms that are expressed differentially in various organs and during development. Overexpression of atSRp30 via a strong constitutive promoter resulted in changes in alternative splicing of several endogenous plant genes, including atSRp30 itself. Interestingly, atSRp30 overexpression resulted in a pronounced down-regulation of endogenous mRNA encoding full-length atSRp34/SR1 protein. Transgenic plants overexpressing atSRp30 showed morphological and developmental changes affecting mostly developmental phase transitions. atSRp30- and atSRp34/SR1-promoter-GUS constructs exhibited complementary expression patterns during early seedling development and root formation, with overlapping expression in floral tissues. The results of the structural and expression analyses of both genes suggest that atSRp34/SR1 acts as a general splicing factor, whereas atSRp30 functions as a specific splicing modulator.

Item Type:	Paper
Uncontrolled Keywords:	SR proteins alternative splicing pre-mRNA processing plant development overexpression phenotype pre-messenger-rna small nuclear ribonucleoprotein sr proteins premessenger rna conserved family site selection 5'-splice-site recognition subnuclear localization distinct functions enhancer complex
Subjects:	organism description > plant > Arabidopsis bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing organism description > plant bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
CSHL Authors:	Kobayashi, Ryuji Krainer, Adrian R.
Communities:	CSHL labs > Kobayashi lab CSHL labs > Krainer lab
Depositing User:	Matt Covey
Date:	April 1999
Date Deposited:	11 Dec 2013 17:48
Last Modified:	11 Dec 2013 17:48
PMCID:	PMC316644
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/28925

Actions (login required)

Administrator's edit/view item