Lippman, Z., May, B., Yordan, C., Singer, T., Martienssen, R.
(December 2003)
Distinct mechanisms determine transposon inheritance and methylation via small interfering RNA and histone modification.
PLoS biology, 1 (3).
pp. 420-428.
ISSN 1545-7885 (Electronic)1544-9173 (Linking)
Abstract
Heritable, but reversible, changes in transposable element activity were first observed in maize by Barbara McClintock in the 1950s. More recently, transposon silencing has been associated with DNA methylation, histone H3 lysine-9 methylation (H3mK9), and RNA interference (RNAi). Using a genetic approach, we have investigated the role of these modifications in the epigenetic regulation and inheritance of six Arabidopsis transposons. Silencing of most of the transposons is relieved in DNA methyltransferase (met1), chromatin remodeling ATPase (ddm1), and histone modification (sil1) mutants. In contrast, only a small subset of the transposons require the H3mK9 methyltransferase KRYPTONITE, the RNAi gene ARGONAUTE1, and the CXG methyltransferase CHROMOMETHYLASE3. In crosses to wild-type plants, epigenetic inheritance of active transposons varied from mutant to mutant, indicating these genes differ in their ability to silence transposons. According to their pattern of transposon regulation, the mutants can be divided into two groups, which suggests that there are distinct, but interacting, complexes or pathways involved in transposon silencing. Furthermore, different transposons tend to be susceptible to different forms of epigenetic regulation.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Adenosine Triphosphatases genetics
Arabidopsis genetics
Arabidopsis Proteins genetics metabolism
Argonaute Proteins
Chromatin metabolism
Chromatin Immunoprecipitation
Computational Biology
DNA (Cytosine-5-)-Methyltransferase genetics
DNA Methylation
DNA Primers chemistry
DNA Transposable Elements
DNA-Binding Proteins metabolism
DNA-Cytosine Methylases
Gene Expression Regulation Plant
Gene Silencing
Genes Plant
Histone-Lysine N-Methyltransferase genetics
Histones metabolism
Models Genetic
Mutation
Open Reading Frames
Polymerase Chain Reaction
RNA Interference
RNA Small Interfering genetics
Transcription Factors metabolism
Transcription Genetic |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Lippman lab
CSHL labs > Martienssen lab
School of Biological Sciences > Publications |
| Depositing User: |
Matt Covey
|
| Date: |
December 2003 |
| Date Deposited: |
27 Mar 2013 16:47 |
| Last Modified: |
19 Sep 2014 14:43 |
| PMCID: |
PMC300680 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/28024 |
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