A role of topoisomerase II in linking DNA replication to chromosome condensation

Cuvier, O., Hirano, T. (March 2003) A role of topoisomerase II in linking DNA replication to chromosome condensation. Journal of Cell Biology, 160 (5). pp. 645-655. ISSN 0021-9525

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Abstract

The condensin complex and topoisomerase 11 (topo 11) have different biochemical activities in vitro, and both are required for mitotic chromosome condensation. We have used Xenopus egg extracts to investigate the functional interplay between condensin and topo 11 in chromosome condensation. When unreplicated chromatin is directly converted into chromosomes with single chromatids, the two proteins must function together, although they are independently targeted to chromosomes. In contrast, the requirement for topo 11 is temporarily separable from that of condensin when chromosome assembly is induced after DNA replication. This experimental setting allows us to find that, in the absence of conclensin, topo 11 becomes enriched in an axial structure within uncondensed chromatin. Subsequent addition of conclensin converts this structure- into mitotic chromosomes in an ATP hydrolysis-dependent manner. Strikingly, preventing DNA replication by the addition of geminin or aphidicolin disturbs the formation of topo II-containing axes and alters the binding property of topo 11 with chromatin. Our results suggest that topo 11 plays an important role in an early stage of chromosome condensation, and that this function of topo 11 is tightly coupled with prior DNA replication.

Item Type:	Paper
Uncontrolled Keywords:	Xenopus egg extracts condensin decatenation compaction SMC XENOPUS EGG EXTRACTS NON-SMC SUBUNITS CHROMATID SEGREGATION MITOTIC CHROMOSOMES PROTEIN COMPLEXES MAMMALIAN-CELLS 13S CONDENSIN MITOSIS SEPARATION METAPHASE
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome organism description > animal > Frog > xenopus
CSHL Authors:	Hirano, Tatsuya Cuvier, Olivier
Communities:	CSHL labs > Hirano lab
Depositing User:	Matt Covey
Date:	March 2003
Date Deposited:	01 Jul 2013 15:58
Last Modified:	01 Jul 2013 15:58
PMCID:	PMC2173373
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27889

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