Diao, A., Rahman, D., Pappin, D. J., Lucocq, J., Lowe, M. (January 2003) The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation. Journal of Cell Biology, 160 (2). pp. 201-12. ISSN 0021-9525
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Abstract
Fragmentation of the mammalian Golgi apparatus during mitosis requires the phosphorylation of a specific subset of Golgi-associated proteins. We have used a biochemical approach to characterize these proteins and report here the identification of golgin-84 as a novel mitotic target. Using cryoelectron microscopy we could localize golgin-84 to the cis-Golgi network and found that it is enriched on tubules emanating from the lateral edges of, and often connecting, Golgi stacks. Golgin-84 binds to active rab1 but not cis-Golgi matrix proteins. Overexpression or depletion of golgin-84 results in fragmentation of the Golgi ribbon. Strikingly, the Golgi ribbon is converted into mini-stacks constituting only approximately 25% of the volume of a normal Golgi apparatus upon golgin-84 depletion. These mini-stacks are able to carry out protein transport, though with reduced efficiency compared with a normal Golgi apparatus. Our results suggest that golgin-84 plays a key role in the assembly and maintenance of the Golgi ribbon in mammalian cells.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Autoantigens Endoplasmic Reticulum/metabolism/ultrastructure Eukaryotic Cells/ metabolism/ultrastructure Gene Expression Regulation/physiology Golgi Apparatus/ metabolism/ultrastructure HeLa Cells Humans Intracellular Membranes/ metabolism/ultrastructure Membrane Proteins/ metabolism/ultrastructure Microscopy, Electron Microtubule Proteins/ metabolism/ultrastructure Mitosis/genetics Phosphoproteins/metabolism Protein Transport/genetics RNA, Small Interfering/diagnostic use Rats Recombinant Fusion Proteins/diagnostic use Subcellular Fractions Viral Proteins/diagnostic use rab1 GTP-Binding Proteins/ metabolism/ultrastructure |
| Subjects: | physics > biophysics > membrane dynamics organs, tissues, organelles, cell types and functions > tissues types and functions > membranes organs, tissues, organelles, cell types and functions > tissues types and functions |
| CSHL Authors: | |
| Communities: | CSHL labs > Pappin lab |
| Depositing User: | Matt Covey |
| Date: | 20 January 2003 |
| Date Deposited: | 01 Jul 2013 19:47 |
| Last Modified: | 01 Jul 2013 19:47 |
| PMCID: | PMC2172652 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27876 |
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