Kato, H., Goto, D. B., Martienssen, R. A., Urano, T., Furukawa, K., Murakami, Y.
(2005)
RNA polymerase II is required for RNAi-dependent heterochromatin assembly.
Science, 309 (5733).
pp. 467-469.
ISSN 00368075 (ISSN)
Abstract
In Schizosaccharomyces pombe, the RNA interference (RNAi) machinery converts pericentromeric transcripts into small interfering RNAs (siRNAs) and is required for the assembly of pericentromeric heterochromatin. Here we describe a mutation in the second largest subunit of RNA polymerase II (RNAPII). Both wild-type and mutant RNAPII localized to the pericentromere. However, the mutation resulted in the loss of heterochromatic histone modifications and in the accumulation of pericentromeric transcripts, accompanied by the loss of siRNAs. This phenotype resembles mutants in RNAi and suggests that RNAPII couples pericentromeric transcription with siRNA processing and heterochromatin assembly.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Polymers
RNA
Surface treatment
Pericentromeric transcription
RNA interference (RNAi)
RNA polymerase II (RNAPII)
Small interfering RNAs (siRNA)
Molecular biology
histone
RNA polymerase II
small interfering RNA
article
centromere
enzyme localization
enzyme subunit
heterochromatin
phenotype
priority journal
protein modification
RNA interference
RNA transcription
Schizosaccharomyces pombe
wild type |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Martienssen lab |
| Depositing User: |
Matt Covey
|
| Date: |
2005 |
| Date Deposited: |
01 Mar 2013 21:50 |
| Last Modified: |
01 Mar 2013 21:50 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/27675 |
Actions (login required)
 |
Administrator's edit/view item |
