nhl-2 Modulates microRNA activity in Caenorhabditis elegans

Hammell, C. M., Lubin, I., Boag, P. R., Blackwell, T. K., Ambros, V. (March 2009) nhl-2 Modulates microRNA activity in Caenorhabditis elegans. Cell, 136 (5). pp. 926-38. ISSN 1097-4172 (Electronic)0092-8674 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/19269369
DOI: 10.1016/j.cell.2009.01.053

Abstract

TRIM-NHL proteins represent a large class of metazoan proteins implicated in development and disease. We demonstrate that a C. elegans TRIM-NHL protein, NHL-2, functions as a cofactor for the microRNA-induced silencing complex (miRISC) and thereby enhances the posttranscriptional repression of several genetically verified microRNA targets, including hbl-1 and let-60/Ras (by the let-7 family of microRNAs) and cog-1 (by the lsy-6 microRNA). NHL-2 is localized to cytoplasmic P-bodies and physically associates with the P-body protein CGH-1 and the core miRISC components ALG-1/2 and AIN-1. nhl-2 and cgh-1 mutations compromise the repression of microRNA targets in vivo but do not affect microRNA biogenesis, indicating a role for an NHL-2:CGH-1 complex in the effector phase of miRISC activity. We propose that the NHL-2:CGH-1 complex functions in association with mature miRISC to modulate the efficacy of microRNA:target interactions in response to physiological and developmental signals, thereby ensuring the robustness of genetic regulatory pathways regulated by microRNAs.

Item Type: Paper
Uncontrolled Keywords: Animals Caenorhabditis elegans/ embryology/ metabolism Caenorhabditis elegans Proteins/genetics/ metabolism Carrier Proteins/genetics/ metabolism Cytoplasmic Granules/metabolism Homeodomain Proteins/metabolism MicroRNAs/ metabolism RNA Nucleotidyltransferases/genetics/metabolism RNA, Helminth/ metabolism RNA-Induced Silencing Complex/metabolism
Subjects: organism description > animal > C elegans
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > animal
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
CSHL Authors:
Communities: CSHL labs > Hammell C. lab
Depositing User: Matt Covey
Date: 6 March 2009
Date Deposited: 20 Feb 2013 15:51
Last Modified: 06 Apr 2015 20:22
PMCID: PMC2670343
Related URLs:
URI: https://repository.cshl.edu/id/eprint/27425

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving