Hammell, C. M., Lubin, I., Boag, P. R., Blackwell, T. K., Ambros, V. (March 2009) nhl-2 Modulates microRNA activity in Caenorhabditis elegans. Cell, 136 (5). pp. 926-38. ISSN 1097-4172 (Electronic)0092-8674 (Linking)
Abstract
TRIM-NHL proteins represent a large class of metazoan proteins implicated in development and disease. We demonstrate that a C. elegans TRIM-NHL protein, NHL-2, functions as a cofactor for the microRNA-induced silencing complex (miRISC) and thereby enhances the posttranscriptional repression of several genetically verified microRNA targets, including hbl-1 and let-60/Ras (by the let-7 family of microRNAs) and cog-1 (by the lsy-6 microRNA). NHL-2 is localized to cytoplasmic P-bodies and physically associates with the P-body protein CGH-1 and the core miRISC components ALG-1/2 and AIN-1. nhl-2 and cgh-1 mutations compromise the repression of microRNA targets in vivo but do not affect microRNA biogenesis, indicating a role for an NHL-2:CGH-1 complex in the effector phase of miRISC activity. We propose that the NHL-2:CGH-1 complex functions in association with mature miRISC to modulate the efficacy of microRNA:target interactions in response to physiological and developmental signals, thereby ensuring the robustness of genetic regulatory pathways regulated by microRNAs.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Caenorhabditis elegans/ embryology/ metabolism Caenorhabditis elegans Proteins/genetics/ metabolism Carrier Proteins/genetics/ metabolism Cytoplasmic Granules/metabolism Homeodomain Proteins/metabolism MicroRNAs/ metabolism RNA Nucleotidyltransferases/genetics/metabolism RNA, Helminth/ metabolism RNA-Induced Silencing Complex/metabolism |
| Subjects: | organism description > animal > C elegans bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organism description > animal bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Hammell C. lab |
| Depositing User: | Matt Covey |
| Date: | 6 March 2009 |
| Date Deposited: | 20 Feb 2013 15:51 |
| Last Modified: | 06 Apr 2015 20:22 |
| PMCID: | PMC2670343 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27425 |
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